Characterization of bacterial communities at heavy-metal-contaminated sites

The microbial community in soil samples from two long-term contaminated sites was characterized by using culture-dependent and culture-independent methods. The two sites investigated contained high amounts of heavy metals and were located in the upper Silesia Industrial Region in southern Poland. The evaluation of the aerobic soil microbial population clearly demonstrated the presence of considerable numbers of viable, culturable bacteria at both sites. A high fraction of the bacterial population was able to grow in the presence of high amounts of metals, i.e. up to 10 mM Zn²⁺, 3 mM Pb²⁺ or 1 mM Cu²⁺. Site 1 contained significantly (P < 0.05) lower bacterial numbers growing in the presence of 10 mM Zn²⁺ than site 2, while the opposite was observed for bacteria tolerating 1 mM Cu²⁺. This coincided with the contents of these two metals at the two sites. Ecophysiological (EP) indices for copiotrophs (r-strategists) and oligotrophs (K-strategists) pointed to high bacterial diversity at both sites. Fluorescence in situ hybridization (FISH) analysis indicated that Actinobacteria and Proteobacteria represent the physiologically active fraction of bacteria at the two sites. Shannon diversity (H′) indices for FISH-detected bacterial phylogenetic groups were not significantly different at the two sites.     

基于LMDI的我国能源消费因素分解研究

经济飞速发展使我国能源消费急剧增长，能源消费与能源供给、环境污染矛盾日益突出。在分析我国能源消费和能源消费强度变化趋势的基础上，运用LMDI法将我国1996--2010年能源消费变化分解为产出效应、结构效应和强度效应，探讨产出提高、结构调整、效率提高对我国能源消费和能源强度的影响。结果显示，生产规模扩大对能源消费起到正向的拉动作用，结构调整对缓解能源消费增长还有较大的上升空间，能源强度对能源消费的抑制效应自“十一五”期间起作用逐步显现。     

大型社会活动安全风险评估指标研究

在对大型社会活动特点及其活动增长趋势分析的基础上,通过对大型社会活动典型伤亡事故的统计,将事故发生直接原因归纳为突发自然灾害、设备设施故障、管控措施失误、疏散设施不符合要求、人为事件和其他因素6类;根据风险管理理论,针对大型社会活动安全风险,从人-机-环-管安全系统工程的观点出发,以准确反映大型社会活动的本质特征和事故预防与控制为目标、尽可能量化为原则,提出具有4个一级指标、9个二级指标和24个三级指标的大型社会活动安全风险评估指标体系和评估依据,并对周边环境、人群密度、疏散速度及人群状况指标进行量化分级。     

Study on Community Diversity of Coastal Shelter Forest in Jiaonan

Evaluating the biodiversity of previous and current species in a forest community is an important task. Some methodological researches and effective applications have been carried out widely in the temperate upland zone. However, they lack related researches in coastal shelter forests. This paper attempts to analyze them for Jiaonan coastal forest communities from the following three aspects, the relationship between plant biodiversity structure and its environment, the nexus between biodivers…     

非突出煤层升级方法和指标体系的探讨

以淮南矿业集团B8煤为研究对象,针对《煤矿瓦斯防治经验五十条》关于非突出煤层升级的一些规定和指标确定的难点,通过对实际突出点的瓦斯地质条件调查分析、现场跟踪考察和实验室实验,重点考察和分析煤层突出危险性与地质构造、构造软煤厚度及瓦斯赋存的关系,结合日常生产中容易获取的各参数,构建了非突出煤层升级的方法和指标体系。该体系中:区域升级指标以相对较容易获取的煤层瓦斯含量和软煤厚度为主要指标;以f,△P值为经常检验的辅助指标;动态监测指标包括:地质构造异常带、构造软煤厚度的变化,日常预测参数K1值、S值,并确定了各指标临界值和应用方法。     

煤中掺比伴生硫化物的自燃特性分析

为了深入探究矿井下伴生硫化物对煤自燃及着火燃烧特性的影响，向原煤中添加不同量的含硫物配制4种不同含硫量的煤样，通过TG实验、DSC测试和XRD分析，研究伴生硫化物对煤自燃及着火燃烧特性的影响规律；基于Coats-Redfern法计算煤中掺加不同伴生硫化物时煤燃烧阶段的活化能。研究结果表明:随着煤中掺比伴生硫化物的增多，煤的特征温度相应减小，而吸氧量、可燃和稳燃指数相应增大，原煤中混入伴生硫化物后更易自燃；随着煤中掺比伴生硫化物的增多，煤燃烧阶段的活化能降低，煤更易着火燃烧；伴生硫化物的主要成分为水绿矾、叶绿矾，这些物质在常温下遇水和氧气能够发生化学循环反应，反应放热促使了煤更易自燃；伴生硫化物在温度高于200℃以后整体表现为放热，在温度为565℃时达到放热峰值，这使得煤燃烧阶段的活化能降低，煤更易燃烧。     

南苕溪支流锦溪水质时空变化特征分析

综合运用灰色聚类法、综合污染指数法、等级聚类法就南苕溪支流锦溪近年水质时空变化特征进行分析。结果表明,锦溪入青山水库断面水质2005—2009年逐年改善,2007年后达到灰色聚类等级Ⅲ类水质要求;2009年秋季至2010年夏季期间秋、冬两季的污染程度高于春、夏季节;枯水期综合污染指数最高,但TN、TP污染分指数在丰水期最高,表明污染源中面源污染已占相当比例。空间变化方面从玲珑桥到江桥锦溪水质的综合污染指数不断提升,到江桥时达到极值13.25,锦溪末端三眼桥断面水质较江桥断面有所改善;SPSS空间聚类分析可分为轻污染区(从玲珑桥到冬韵桥)、重污染区(从群利桥到江桥)和中度污染区(从江桥到三眼桥)。锦溪上游农业区对河水中的COD、TN和TP的贡献率分别为66%、54%和49%,农业面源污染已成为制约锦溪水质改善的主要因素。     

Genotoxicity assessment of human peripheral Lymphocytes induced by thallium(I) and thallium(III)

Thallium is a non-essential metal with a wide range of industrial uses. However, thallium is also a potential pollutant with high potential toxicity to humans. In the present study, we analyzed and compared the cellular and genotoxic effects of thallium in two main oxidation states by applying chromosome aberration assays to human peripheral lymphocytes. We observed that thallium(I) sulfate reduced the mitotic index at all tested concentrations (0.5, 1, 5, 50 and 100 μg/mL), whereas thallium(III) chloride was toxic at concentrations ≥1 μg/mL. Thallium(I) and thallium(III) treatment significantly increased structural chromosomal aberrations, with and without gaps, and increased the percentage of aberrant cells without gaps. Furthermore, satellite associations and numerical chromosomal aberration tests showed significant differences at a few of the tested concentrations. The satellite association test is related to aneuploidy. Thallium salts increased satellite associations when hyperploid cells were observed. Our results indicated that the two oxidation states of thallium induced toxicity in vitro – i.e. cyto/genotoxic (clastogenic and aneuploidogenic) effects.     

Enhancing the Ability of a Soil Moisture‐based Index for Agricultural Drought Monitoring by Incorporating Root Distribution

Agricultural drought differs from meteorological, hydrological, and socioeconomic drought, being closely related to soil water availability in the root zone, specifically for crop and crop growth stage. In previous studies, several soil moisture indices (e.g., the soil moisture index, soil water deficit index) based on soil water availability have been developed for agricultural drought monitoring. However, when developing these indices, it was generally assumed that soil water availability to crops was equal throughout the root zone, and the effects of root distribution and crop growth stage on soil water uptake were ignored. This article aims to incorporate root distribution into a soil moisture‐based index and to evaluate the performance of the improved soil moisture index for agricultural drought monitoring. The Huang‐Huai‐Hai Plain of China was used as the study area. Overall, soil moisture indices were significantly correlated with the crop moisture index (CMI), and the improved root‐weighted soil moisture index (RSMI) was more closely related to the CMI than averaged soil moisture indices. The RSMI correctly identified most of the observed drought events and performed well in the detection of drought levels. Furthermore, the RSMI had a better performance than averaged soil moisture indices when compared to crop yield. In conclusion, soil moisture indices could improve agricultural drought monitoring by incorporating root distribution.     

近55年来华东地区旱涝时空变化特征

基于华东地区89个气象站点近55年逐月降水资料,运用趋势分析法、IDW空间插值法、标准化降水指数(SPI)等方法,分析了1960~2014年期间我国华东地区旱涝灾害时空变化特征,并探讨了淮河南北旱涝的时空差异。结果表明:(1)近55年华东地区降水量整体呈波动上升趋势,其中淮河以北地区降水量呈波动下降趋势,而淮河以南呈上升趋势。(2)从年尺度旱涝变化看,华东地区雨涝频率大于干旱频率,但干旱强度大于雨涝强度,且淮河以南地区的干旱强度大于淮河以北,而雨涝强度小于淮河以北。(3)华东地区南北旱涝站次比呈反向发展趋势,即淮河以北旱灾影响范围不断扩大,雨涝影响范围不断减小,淮河以南与之相反。(4)华东地区严重旱涝频率在空间分布上呈南北反向格局,即淮河以北旱灾频率高,涝灾频率低;淮河以南涝灾频率高,旱灾频率低。